The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality

Variable screen quality, off-target effects, and unclear false discovery rates often hamper large-scale functional genomic screens in mammalian cells. Hart et al (2014) introduce gold standard reference sets of essential and non-essential genes, aiming at standardizing the analysis of genome-wide sc...

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Autores principales: Evers, Bastiaan, Bernards, Rene, Beijersbergen, Roderick L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2014
Materias:
News & Views
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299495/
https://www.ncbi.nlm.nih.gov/pubmed/24987117
http://dx.doi.org/10.15252/msb.20145372
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author Evers, Bastiaan
Bernards, Rene
Beijersbergen, Roderick L
author_facet Evers, Bastiaan
Bernards, Rene
Beijersbergen, Roderick L
author_sort Evers, Bastiaan
collection PubMed
description Variable screen quality, off-target effects, and unclear false discovery rates often hamper large-scale functional genomic screens in mammalian cells. Hart et al (2014) introduce gold standard reference sets of essential and non-essential genes, aiming at standardizing the analysis of genome-wide screens. This work provides a framework to compare both the quality and analysis methods of functional genetic screens.
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spelling pubmed-42994952015-01-20 The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality Evers, Bastiaan Bernards, Rene Beijersbergen, Roderick L Mol Syst Biol News & Views Variable screen quality, off-target effects, and unclear false discovery rates often hamper large-scale functional genomic screens in mammalian cells. Hart et al (2014) introduce gold standard reference sets of essential and non-essential genes, aiming at standardizing the analysis of genome-wide screens. This work provides a framework to compare both the quality and analysis methods of functional genetic screens. Blackwell Publishing Ltd 2014-07-01 /pmc/articles/PMC4299495/ /pubmed/24987117 http://dx.doi.org/10.15252/msb.20145372 Text en © 2014 The Authors. Published under the terms of the CC BY 4.0 license http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle News & Views
Evers, Bastiaan
Bernards, Rene
Beijersbergen, Roderick L
The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality
title The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality
title_full The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality
title_fullStr The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality
title_full_unstemmed The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality
title_short The Good, the Bad, and the Ugly: in search of gold standards for assessing functional genetic screen quality
title_sort good, the bad, and the ugly: in search of gold standards for assessing functional genetic screen quality
topic News & Views
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299495/
https://www.ncbi.nlm.nih.gov/pubmed/24987117
http://dx.doi.org/10.15252/msb.20145372
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