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Development and validation of an rDNA operon based primer walking strategy applicable to de novo bacterial genome finishing

Advances in sequencing technology have drastically increased the depth and feasibility of bacterial genome sequencing. However, little information is available that details the specific techniques and procedures employed during genome sequencing despite the large numbers of published genomes. Shotgu...

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Detalles Bibliográficos
Autores principales: Eastman, Alexander W., Yuan, Ze-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4301005/
https://www.ncbi.nlm.nih.gov/pubmed/25653642
http://dx.doi.org/10.3389/fmicb.2014.00769