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Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template

Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is. Here we describe an approach in which the first primer binds in a parallel complementary orientation to the single-s...

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Detalles Bibliográficos
Autores principales: Bhardwaj, Vikash, Sharma, Kulbhushan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304224/
https://www.ncbi.nlm.nih.gov/pubmed/25653847
http://dx.doi.org/10.12688/f1000research.5813.1