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Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is. Here we describe an approach in which the first primer binds in a parallel complementary orientation to the single-s...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
F1000Research
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304224/ https://www.ncbi.nlm.nih.gov/pubmed/25653847 http://dx.doi.org/10.12688/f1000research.5813.1 |