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Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template

Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is. Here we describe an approach in which the first primer binds in a parallel complementary orientation to the single-s...

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Detalles Bibliográficos
Autores principales: Bhardwaj, Vikash, Sharma, Kulbhushan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304224/
https://www.ncbi.nlm.nih.gov/pubmed/25653847
http://dx.doi.org/10.12688/f1000research.5813.1
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author Bhardwaj, Vikash
Sharma, Kulbhushan
author_facet Bhardwaj, Vikash
Sharma, Kulbhushan
author_sort Bhardwaj, Vikash
collection PubMed
description Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is. Here we describe an approach in which the first primer binds in a parallel complementary orientation to the single-stranded DNA, leading to synthesis in a parallel direction. Further reactions happened in a conventional way leading to the synthesis of PCR product having polarity opposite to the template used. This is the first study showing that synthesis of DNA can happen also in a parallel direction. We report that from a single-stranded DNA template, two different but related PCR products can be synthesized.
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spelling pubmed-43042242015-02-03 Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template Bhardwaj, Vikash Sharma, Kulbhushan F1000Res Method Article Conventionally, in a polymerase chain reaction (PCR), oligonucleotide primers bind to the template DNA in an antiparallel complementary way and the template DNA is amplified as it is. Here we describe an approach in which the first primer binds in a parallel complementary orientation to the single-stranded DNA, leading to synthesis in a parallel direction. Further reactions happened in a conventional way leading to the synthesis of PCR product having polarity opposite to the template used. This is the first study showing that synthesis of DNA can happen also in a parallel direction. We report that from a single-stranded DNA template, two different but related PCR products can be synthesized. F1000Research 2014-12-31 /pmc/articles/PMC4304224/ /pubmed/25653847 http://dx.doi.org/10.12688/f1000research.5813.1 Text en Copyright: © 2014 Bhardwaj V and Sharma K http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/publicdomain/zero/1.0/ Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).
spellingShingle Method Article
Bhardwaj, Vikash
Sharma, Kulbhushan
Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
title Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
title_full Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
title_fullStr Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
title_full_unstemmed Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
title_short Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template
title_sort parallel dna polymerase chain reaction: synthesis of two different pcr products from a dna template
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304224/
https://www.ncbi.nlm.nih.gov/pubmed/25653847
http://dx.doi.org/10.12688/f1000research.5813.1
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