An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

BACKGROUND: Cell display technologies (e.g. bacterial display) are attractive in directed evolution as they provide the option to use flow-cytometric cell sorting for selection from combinatorial libraries. The aim of this study was to engineer and investigate an expression vector system with dual f...

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Detalles Bibliográficos
Autores principales: Fleetwood, Filippa, Andersson, Ken G, Ståhl, Stefan, Löfblom, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304625/
https://www.ncbi.nlm.nih.gov/pubmed/25547008
http://dx.doi.org/10.1186/s12934-014-0179-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4304625/
https://www.ncbi.nlm.nih.gov/pubmed/25547008
http://dx.doi.org/10.1186/s12934-014-0179-z

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