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Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness
BACKGROUND: We have previously found in the chronic SKG mouse model of arthritis that long standing (5 and 8 months) inflammation directly leads to high collagen bone turnover, disorganization of the collagen network, disturbed bone microstructure and degradation of bone biomechanical properties. Th...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305284/ https://www.ncbi.nlm.nih.gov/pubmed/25617902 http://dx.doi.org/10.1371/journal.pone.0117100 |
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author | Vidal, Bruno Cascão, Rita Vale, Ana Catarina Cavaleiro, Inês Vaz, Maria Fátima Brito, José Américo Almeida Canhão, Helena Fonseca, João Eurico |
author_facet | Vidal, Bruno Cascão, Rita Vale, Ana Catarina Cavaleiro, Inês Vaz, Maria Fátima Brito, José Américo Almeida Canhão, Helena Fonseca, João Eurico |
author_sort | Vidal, Bruno |
collection | PubMed |
description | BACKGROUND: We have previously found in the chronic SKG mouse model of arthritis that long standing (5 and 8 months) inflammation directly leads to high collagen bone turnover, disorganization of the collagen network, disturbed bone microstructure and degradation of bone biomechanical properties. The main goal of the present work was to study the effects of the first days of the inflammatory process on the microarchitecture and mechanical properties of bone. METHODS: Twenty eight Wistar adjuvant-induced arthritis (AIA) rats were monitored during 22 days after disease induction for the inflammatory score, ankle perimeter and body weight. Healthy non-arthritic rats were used as controls for compar-ison. After 22 days of disease progression rats were sacrificed and bone samples were collected for histomorphometrical, energy dispersive X-ray spectroscopical analysis and 3-point bending. Blood samples were also collected for bone turnover markers. RESULTS: AIA rats had an increased bone turnover (as inferred from increased P1NP and CTX1, p = 0.0010 and p = 0.0002, respectively) and this was paralleled by a decreased mineral content (calcium p = 0.0046 and phos-phorus p = 0.0046). Histomorphometry showed a lower trabecular thickness (p = 0.0002) and bone volume (p = 0.0003) and higher trabecular sepa-ration (p = 0.0009) in the arthritic group as compared with controls. In addition, bone mechanical tests showed evidence of fragility as depicted by diminished values of yield stress and ultimate fracture point (p = 0.0061 and p = 0.0279, re-spectively) in the arthritic group. CONCLUSIONS: We have shown in an AIA rat model that arthritis induc-es early bone high turnover, structural degradation, mineral loss and mechanical weak-ness. |
format | Online Article Text |
id | pubmed-4305284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-43052842015-01-30 Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness Vidal, Bruno Cascão, Rita Vale, Ana Catarina Cavaleiro, Inês Vaz, Maria Fátima Brito, José Américo Almeida Canhão, Helena Fonseca, João Eurico PLoS One Research Article BACKGROUND: We have previously found in the chronic SKG mouse model of arthritis that long standing (5 and 8 months) inflammation directly leads to high collagen bone turnover, disorganization of the collagen network, disturbed bone microstructure and degradation of bone biomechanical properties. The main goal of the present work was to study the effects of the first days of the inflammatory process on the microarchitecture and mechanical properties of bone. METHODS: Twenty eight Wistar adjuvant-induced arthritis (AIA) rats were monitored during 22 days after disease induction for the inflammatory score, ankle perimeter and body weight. Healthy non-arthritic rats were used as controls for compar-ison. After 22 days of disease progression rats were sacrificed and bone samples were collected for histomorphometrical, energy dispersive X-ray spectroscopical analysis and 3-point bending. Blood samples were also collected for bone turnover markers. RESULTS: AIA rats had an increased bone turnover (as inferred from increased P1NP and CTX1, p = 0.0010 and p = 0.0002, respectively) and this was paralleled by a decreased mineral content (calcium p = 0.0046 and phos-phorus p = 0.0046). Histomorphometry showed a lower trabecular thickness (p = 0.0002) and bone volume (p = 0.0003) and higher trabecular sepa-ration (p = 0.0009) in the arthritic group as compared with controls. In addition, bone mechanical tests showed evidence of fragility as depicted by diminished values of yield stress and ultimate fracture point (p = 0.0061 and p = 0.0279, re-spectively) in the arthritic group. CONCLUSIONS: We have shown in an AIA rat model that arthritis induc-es early bone high turnover, structural degradation, mineral loss and mechanical weak-ness. Public Library of Science 2015-01-24 /pmc/articles/PMC4305284/ /pubmed/25617902 http://dx.doi.org/10.1371/journal.pone.0117100 Text en © 2015 Vidal et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Vidal, Bruno Cascão, Rita Vale, Ana Catarina Cavaleiro, Inês Vaz, Maria Fátima Brito, José Américo Almeida Canhão, Helena Fonseca, João Eurico Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness |
title | Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness |
title_full | Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness |
title_fullStr | Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness |
title_full_unstemmed | Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness |
title_short | Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness |
title_sort | arthritis induces early bone high turnover, structural degradation and mechanical weakness |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4305284/ https://www.ncbi.nlm.nih.gov/pubmed/25617902 http://dx.doi.org/10.1371/journal.pone.0117100 |
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