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Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves' Ophthalmopathy
Purpose. To evaluate the relationship between the expression of orbital tissue mRNA for FOXP3, CTLA-4/CD28/CD80/CD86, and CD40/CD40 and the severity of Graves' orbitopathy (GO). Material and Methods. Orbital tissue was obtained from 26 patients with GO, with mild (n = 6) or severe GO (n = 20),...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306377/ https://www.ncbi.nlm.nih.gov/pubmed/25653477 http://dx.doi.org/10.1155/2015/340934 |
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author | Pawlowski, Przemyslaw Wawrusiewicz-Kurylonek, Natalia Eckstein, Anja Reszec, Joanna Luczynski, Wlodzimierz Johnson, Kristian Kretowski, Adam Bakunowicz-Lazarczyk, Alina Gorska, Maria Szamatowicz, Jacek Chyczewski, Lech Mysliwiec, Janusz |
author_facet | Pawlowski, Przemyslaw Wawrusiewicz-Kurylonek, Natalia Eckstein, Anja Reszec, Joanna Luczynski, Wlodzimierz Johnson, Kristian Kretowski, Adam Bakunowicz-Lazarczyk, Alina Gorska, Maria Szamatowicz, Jacek Chyczewski, Lech Mysliwiec, Janusz |
author_sort | Pawlowski, Przemyslaw |
collection | PubMed |
description | Purpose. To evaluate the relationship between the expression of orbital tissue mRNA for FOXP3, CTLA-4/CD28/CD80/CD86, and CD40/CD40 and the severity of Graves' orbitopathy (GO). Material and Methods. Orbital tissue was obtained from 26 patients with GO, with mild (n = 6) or severe GO (n = 20), and 7 healthy controls. The expression of mRNA of FOXP3, CTLA-4/CD28/CD80/CD86, CD40/CD40L was measured by RT-PCR. TCR and CD3 were evaluated by immunohistochemistry. Results. Higher mRNA for FoxP3 (relative expression: 1.4) and CD40 (1.27) and lower expression of CTLA-4 (0.61) were found in the GO tissues versus controls. In severe GO as compared to mild GO higher mRNA expression for FoxP3 (1.35) and CD40 (1.4) and lower expression for CTLA-4 (0.78), CD28 (0.62), and CD40L (0.56) were found. A positive correlation was found between FOXP3 mRNA and CD3 infiltration (R = 0.796, P = 0.0000001). Conclusions. The enhanced FOXP3 mRNA expression in GO samples may suggest the dysfunction of FOXP3 cells in the severe GO. The diminished mRNA expression of CTLA-4 in severe GO may indicate inadequate T regulatory function. The enhanced mRNA expression of CD40 in severe GO and negative correlation to CRP mRNA may suggest their role in the active and inactive GO. |
format | Online Article Text |
id | pubmed-4306377 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-43063772015-02-04 Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves' Ophthalmopathy Pawlowski, Przemyslaw Wawrusiewicz-Kurylonek, Natalia Eckstein, Anja Reszec, Joanna Luczynski, Wlodzimierz Johnson, Kristian Kretowski, Adam Bakunowicz-Lazarczyk, Alina Gorska, Maria Szamatowicz, Jacek Chyczewski, Lech Mysliwiec, Janusz Mediators Inflamm Research Article Purpose. To evaluate the relationship between the expression of orbital tissue mRNA for FOXP3, CTLA-4/CD28/CD80/CD86, and CD40/CD40 and the severity of Graves' orbitopathy (GO). Material and Methods. Orbital tissue was obtained from 26 patients with GO, with mild (n = 6) or severe GO (n = 20), and 7 healthy controls. The expression of mRNA of FOXP3, CTLA-4/CD28/CD80/CD86, CD40/CD40L was measured by RT-PCR. TCR and CD3 were evaluated by immunohistochemistry. Results. Higher mRNA for FoxP3 (relative expression: 1.4) and CD40 (1.27) and lower expression of CTLA-4 (0.61) were found in the GO tissues versus controls. In severe GO as compared to mild GO higher mRNA expression for FoxP3 (1.35) and CD40 (1.4) and lower expression for CTLA-4 (0.78), CD28 (0.62), and CD40L (0.56) were found. A positive correlation was found between FOXP3 mRNA and CD3 infiltration (R = 0.796, P = 0.0000001). Conclusions. The enhanced FOXP3 mRNA expression in GO samples may suggest the dysfunction of FOXP3 cells in the severe GO. The diminished mRNA expression of CTLA-4 in severe GO may indicate inadequate T regulatory function. The enhanced mRNA expression of CD40 in severe GO and negative correlation to CRP mRNA may suggest their role in the active and inactive GO. Hindawi Publishing Corporation 2015 2015-01-12 /pmc/articles/PMC4306377/ /pubmed/25653477 http://dx.doi.org/10.1155/2015/340934 Text en Copyright © 2015 Przemyslaw Pawlowski et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Pawlowski, Przemyslaw Wawrusiewicz-Kurylonek, Natalia Eckstein, Anja Reszec, Joanna Luczynski, Wlodzimierz Johnson, Kristian Kretowski, Adam Bakunowicz-Lazarczyk, Alina Gorska, Maria Szamatowicz, Jacek Chyczewski, Lech Mysliwiec, Janusz Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves' Ophthalmopathy |
title | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves' Ophthalmopathy |
title_full | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves' Ophthalmopathy |
title_fullStr | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves' Ophthalmopathy |
title_full_unstemmed | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves' Ophthalmopathy |
title_short | Disturbances of Modulating Molecules (FOXP3, CTLA-4/CD28/B7, and CD40/CD40L) mRNA Expressions in the Orbital Tissue from Patients with Severe Graves' Ophthalmopathy |
title_sort | disturbances of modulating molecules (foxp3, ctla-4/cd28/b7, and cd40/cd40l) mrna expressions in the orbital tissue from patients with severe graves' ophthalmopathy |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306377/ https://www.ncbi.nlm.nih.gov/pubmed/25653477 http://dx.doi.org/10.1155/2015/340934 |
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