The Standard Scrapie Cell Assay: Development, Utility and Prospects

Prion diseases are a family of fatal neurodegenerative diseases that involve the misfolding of a host protein, PrP(C). Measuring prion infectivity is necessary for determining efficacy of a treatment or infectivity of a prion purification procedure; animal bioassays are, however, very expensive and...

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Autores principales: van der Merwe, Jacques, Aiken, Judd, Westaway, David, McKenzie, Debbie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306833/
https://www.ncbi.nlm.nih.gov/pubmed/25602372
http://dx.doi.org/10.3390/v7010180
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author van der Merwe, Jacques
Aiken, Judd
Westaway, David
McKenzie, Debbie
author_facet van der Merwe, Jacques
Aiken, Judd
Westaway, David
McKenzie, Debbie
author_sort van der Merwe, Jacques
collection PubMed
description Prion diseases are a family of fatal neurodegenerative diseases that involve the misfolding of a host protein, PrP(C). Measuring prion infectivity is necessary for determining efficacy of a treatment or infectivity of a prion purification procedure; animal bioassays are, however, very expensive and time consuming. The Standard Scrapie Cell Assay (SSCA) provides an alternative approach. The SSCA facilitates quantitative in vitro analysis of prion strains, titres and biological properties. Given its robust nature and potential for high throughput, the SSCA has substantial utility for in vitro characterization of prions and can be deployed in a number of settings. Here we provide an overview on establishing the SSCA, its use in studies of disease dissemination and pathogenesis, potential pitfalls and a number of remaining challenges.
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spelling pubmed-43068332015-02-02 The Standard Scrapie Cell Assay: Development, Utility and Prospects van der Merwe, Jacques Aiken, Judd Westaway, David McKenzie, Debbie Viruses Review Prion diseases are a family of fatal neurodegenerative diseases that involve the misfolding of a host protein, PrP(C). Measuring prion infectivity is necessary for determining efficacy of a treatment or infectivity of a prion purification procedure; animal bioassays are, however, very expensive and time consuming. The Standard Scrapie Cell Assay (SSCA) provides an alternative approach. The SSCA facilitates quantitative in vitro analysis of prion strains, titres and biological properties. Given its robust nature and potential for high throughput, the SSCA has substantial utility for in vitro characterization of prions and can be deployed in a number of settings. Here we provide an overview on establishing the SSCA, its use in studies of disease dissemination and pathogenesis, potential pitfalls and a number of remaining challenges. MDPI 2015-01-16 /pmc/articles/PMC4306833/ /pubmed/25602372 http://dx.doi.org/10.3390/v7010180 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
van der Merwe, Jacques
Aiken, Judd
Westaway, David
McKenzie, Debbie
The Standard Scrapie Cell Assay: Development, Utility and Prospects
title The Standard Scrapie Cell Assay: Development, Utility and Prospects
title_full The Standard Scrapie Cell Assay: Development, Utility and Prospects
title_fullStr The Standard Scrapie Cell Assay: Development, Utility and Prospects
title_full_unstemmed The Standard Scrapie Cell Assay: Development, Utility and Prospects
title_short The Standard Scrapie Cell Assay: Development, Utility and Prospects
title_sort standard scrapie cell assay: development, utility and prospects
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306833/
https://www.ncbi.nlm.nih.gov/pubmed/25602372
http://dx.doi.org/10.3390/v7010180
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