The Standard Scrapie Cell Assay: Development, Utility and Prospects
Prion diseases are a family of fatal neurodegenerative diseases that involve the misfolding of a host protein, PrP(C). Measuring prion infectivity is necessary for determining efficacy of a treatment or infectivity of a prion purification procedure; animal bioassays are, however, very expensive and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306833/ https://www.ncbi.nlm.nih.gov/pubmed/25602372 http://dx.doi.org/10.3390/v7010180 |
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author | van der Merwe, Jacques Aiken, Judd Westaway, David McKenzie, Debbie |
author_facet | van der Merwe, Jacques Aiken, Judd Westaway, David McKenzie, Debbie |
author_sort | van der Merwe, Jacques |
collection | PubMed |
description | Prion diseases are a family of fatal neurodegenerative diseases that involve the misfolding of a host protein, PrP(C). Measuring prion infectivity is necessary for determining efficacy of a treatment or infectivity of a prion purification procedure; animal bioassays are, however, very expensive and time consuming. The Standard Scrapie Cell Assay (SSCA) provides an alternative approach. The SSCA facilitates quantitative in vitro analysis of prion strains, titres and biological properties. Given its robust nature and potential for high throughput, the SSCA has substantial utility for in vitro characterization of prions and can be deployed in a number of settings. Here we provide an overview on establishing the SSCA, its use in studies of disease dissemination and pathogenesis, potential pitfalls and a number of remaining challenges. |
format | Online Article Text |
id | pubmed-4306833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-43068332015-02-02 The Standard Scrapie Cell Assay: Development, Utility and Prospects van der Merwe, Jacques Aiken, Judd Westaway, David McKenzie, Debbie Viruses Review Prion diseases are a family of fatal neurodegenerative diseases that involve the misfolding of a host protein, PrP(C). Measuring prion infectivity is necessary for determining efficacy of a treatment or infectivity of a prion purification procedure; animal bioassays are, however, very expensive and time consuming. The Standard Scrapie Cell Assay (SSCA) provides an alternative approach. The SSCA facilitates quantitative in vitro analysis of prion strains, titres and biological properties. Given its robust nature and potential for high throughput, the SSCA has substantial utility for in vitro characterization of prions and can be deployed in a number of settings. Here we provide an overview on establishing the SSCA, its use in studies of disease dissemination and pathogenesis, potential pitfalls and a number of remaining challenges. MDPI 2015-01-16 /pmc/articles/PMC4306833/ /pubmed/25602372 http://dx.doi.org/10.3390/v7010180 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review van der Merwe, Jacques Aiken, Judd Westaway, David McKenzie, Debbie The Standard Scrapie Cell Assay: Development, Utility and Prospects |
title | The Standard Scrapie Cell Assay: Development, Utility and Prospects |
title_full | The Standard Scrapie Cell Assay: Development, Utility and Prospects |
title_fullStr | The Standard Scrapie Cell Assay: Development, Utility and Prospects |
title_full_unstemmed | The Standard Scrapie Cell Assay: Development, Utility and Prospects |
title_short | The Standard Scrapie Cell Assay: Development, Utility and Prospects |
title_sort | standard scrapie cell assay: development, utility and prospects |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306833/ https://www.ncbi.nlm.nih.gov/pubmed/25602372 http://dx.doi.org/10.3390/v7010180 |
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