Effect of the enzyme and PCR conditions on the quality of high-throughput DNA sequencing results

Library preparation protocols for high-throughput DNA sequencing (HTS) include amplification steps in which errors can build up. In order to have confidence in the sequencing data, it is important to understand the effects of different Taq polymerases and PCR amplification protocols on the DNA molec...

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Detalles Bibliográficos
Autores principales: Brandariz-Fontes, Claudia, Camacho-Sanchez, Miguel, Vilà, Carles, Vega-Pla, José Luis, Rico, Ciro, Leonard, Jennifer A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306961/
https://www.ncbi.nlm.nih.gov/pubmed/25623996
http://dx.doi.org/10.1038/srep08056

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4306961/
https://www.ncbi.nlm.nih.gov/pubmed/25623996
http://dx.doi.org/10.1038/srep08056

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