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Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples

BACKGROUND: Detection and quantification of plant pathogens in the presence of inhibitory substances can be a challenge especially with plant and environmental samples. Real-time quantitative PCR has enabled high-throughput detection and quantification of pathogens; however, its quantitative use is...

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Detalles Bibliográficos
Autores principales: Rački, Nejc, Dreo, Tanja, Gutierrez-Aguirre, Ion, Blejec, Andrej, Ravnikar, Maja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307183/
https://www.ncbi.nlm.nih.gov/pubmed/25628753
http://dx.doi.org/10.1186/s13007-014-0042-6