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Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples
BACKGROUND: Detection and quantification of plant pathogens in the presence of inhibitory substances can be a challenge especially with plant and environmental samples. Real-time quantitative PCR has enabled high-throughput detection and quantification of pathogens; however, its quantitative use is...
Autores principales: | Rački, Nejc, Dreo, Tanja, Gutierrez-Aguirre, Ion, Blejec, Andrej, Ravnikar, Maja |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307183/ https://www.ncbi.nlm.nih.gov/pubmed/25628753 http://dx.doi.org/10.1186/s13007-014-0042-6 |
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