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Development and Application of a Label-Free Fluorescence Method for Determining the Composition of Gold Nanoparticle–Protein Conjugates

A method was developed for determining the composition of the conjugates between gold nanoparticles and proteins based on the intrinsic fluorescence of unbound protein molecules. The fluorescence was evaluated after separation of the conjugates from the reaction mixture by centrifugation. Gold nanop...

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Detalles Bibliográficos
Autores principales: Sotnikov, Dmitriy V., Zherdev, Anatoly V., Dzantiev, Boris B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307282/
https://www.ncbi.nlm.nih.gov/pubmed/25561238
http://dx.doi.org/10.3390/ijms16010907