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Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli

BACKGROUND: Escherichia coli is commonly used in academia and industry for expressing recombinant proteins because of its well-characterized molecular genetics and the availability of numerous expression vectors and strains. One important issue during recombinant protein production is the so-called...

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Detalles Bibliográficos
Autores principales: Rahmen, Natalie, Fulton, Alexander, Ihling, Nina, Magni, Marzio, Jaeger, Karl-Erich, Büchs, Jochen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307990/
https://www.ncbi.nlm.nih.gov/pubmed/25612616
http://dx.doi.org/10.1186/s12934-015-0191-y