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Exchange of single amino acids at different positions of a recombinant protein affects metabolic burden in Escherichia coli

BACKGROUND: Escherichia coli is commonly used in academia and industry for expressing recombinant proteins because of its well-characterized molecular genetics and the availability of numerous expression vectors and strains. One important issue during recombinant protein production is the so-called...

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Detalles Bibliográficos
Autores principales:	Rahmen, Natalie, Fulton, Alexander, Ihling, Nina, Magni, Marzio, Jaeger, Karl-Erich, Büchs, Jochen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4307990/ https://www.ncbi.nlm.nih.gov/pubmed/25612616 http://dx.doi.org/10.1186/s12934-015-0191-y

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