Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells

This study investigated the effects of an ethanol extract of black pepper and its constituent, piperine, on odorant-induced signal transduction in non-chemosensory cells. An ethanol extract of black pepper decreased eugenol-induced cAMP and calcium levels in preadipocyte 3T3-L1 cells with no toxicit...

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Autores principales: Yoon, Yeo Cho, Kim, Sung-Hee, Kim, Min Jung, Yang, Hye Jeong, Rhyu, Mee-Ra, Park, Jae-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309837/
https://www.ncbi.nlm.nih.gov/pubmed/25685661
http://dx.doi.org/10.1016/j.fob.2014.11.008
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author Yoon, Yeo Cho
Kim, Sung-Hee
Kim, Min Jung
Yang, Hye Jeong
Rhyu, Mee-Ra
Park, Jae-Ho
author_facet Yoon, Yeo Cho
Kim, Sung-Hee
Kim, Min Jung
Yang, Hye Jeong
Rhyu, Mee-Ra
Park, Jae-Ho
author_sort Yoon, Yeo Cho
collection PubMed
description This study investigated the effects of an ethanol extract of black pepper and its constituent, piperine, on odorant-induced signal transduction in non-chemosensory cells. An ethanol extract of black pepper decreased eugenol-induced cAMP and calcium levels in preadipocyte 3T3-L1 cells with no toxicity. Phosphorylation of CREB (cAMP response element-binding protein) was down-regulated by the black pepper extract. The concentration (133.8 mg/g) and retention time (5.5 min) of piperine in the ethanol extract were quantified using UPLC–MS/MS. Pretreatment with piperine decreased eugenol-induced cAMP and calcium levels in 3T3-L1 cells. Piperine also decreased the phosphorylation of CREB, which is up-regulated by eugenol. These results suggest that piperine inhibits the eugenol-induced signal transduction pathway through modulation of cAMP and calcium levels and phosphorylation of CREB in non-chemosensory cells.
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spelling pubmed-43098372015-02-14 Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells Yoon, Yeo Cho Kim, Sung-Hee Kim, Min Jung Yang, Hye Jeong Rhyu, Mee-Ra Park, Jae-Ho FEBS Open Bio Article This study investigated the effects of an ethanol extract of black pepper and its constituent, piperine, on odorant-induced signal transduction in non-chemosensory cells. An ethanol extract of black pepper decreased eugenol-induced cAMP and calcium levels in preadipocyte 3T3-L1 cells with no toxicity. Phosphorylation of CREB (cAMP response element-binding protein) was down-regulated by the black pepper extract. The concentration (133.8 mg/g) and retention time (5.5 min) of piperine in the ethanol extract were quantified using UPLC–MS/MS. Pretreatment with piperine decreased eugenol-induced cAMP and calcium levels in 3T3-L1 cells. Piperine also decreased the phosphorylation of CREB, which is up-regulated by eugenol. These results suggest that piperine inhibits the eugenol-induced signal transduction pathway through modulation of cAMP and calcium levels and phosphorylation of CREB in non-chemosensory cells. Elsevier 2014-11-27 /pmc/articles/PMC4309837/ /pubmed/25685661 http://dx.doi.org/10.1016/j.fob.2014.11.008 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).
spellingShingle Article
Yoon, Yeo Cho
Kim, Sung-Hee
Kim, Min Jung
Yang, Hye Jeong
Rhyu, Mee-Ra
Park, Jae-Ho
Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells
title Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells
title_full Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells
title_fullStr Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells
title_full_unstemmed Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells
title_short Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells
title_sort piperine, a component of black pepper, decreases eugenol-induced camp and calcium levels in non-chemosensory 3t3-l1 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4309837/
https://www.ncbi.nlm.nih.gov/pubmed/25685661
http://dx.doi.org/10.1016/j.fob.2014.11.008
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