Cargando…

Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat

As a class of water-soluble, fructose-based oligo- and polysaccharides, fructans are major nonstructural carbohydrates and an important carbon source for grain filling in wheat (Triticum aestivum L.). Four enzymes are involved in fructan synthesis in higher plants, and 6-SFT is a key enzyme in fruct...

Descripción completa

Detalles Bibliográficos
Autores principales: Yue, Aiqin, Li, Ang, Mao, Xinguo, Chang, Xiaoping, Li, Runzhi, Jing, Ruilian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311048/
https://www.ncbi.nlm.nih.gov/pubmed/25653572
http://dx.doi.org/10.1007/s11032-015-0266-9
_version_ 1782354945196949504
author Yue, Aiqin
Li, Ang
Mao, Xinguo
Chang, Xiaoping
Li, Runzhi
Jing, Ruilian
author_facet Yue, Aiqin
Li, Ang
Mao, Xinguo
Chang, Xiaoping
Li, Runzhi
Jing, Ruilian
author_sort Yue, Aiqin
collection PubMed
description As a class of water-soluble, fructose-based oligo- and polysaccharides, fructans are major nonstructural carbohydrates and an important carbon source for grain filling in wheat (Triticum aestivum L.). Four enzymes are involved in fructan synthesis in higher plants, and 6-SFT is a key enzyme in fructan biosynthesis. In this study, thirteen single nucleotide polymorphisms were detected in 6-SFT-A2 in 24 wheat accessions, forming three haplotypes. Two cleaved amplified polymorphic sequence markers developed based on polymorphisms at sites 1870(A-G) and 1951(A-G) distinguished the three haplotypes. 6-SFT-A2 was located on chromosome 4A, between markers P2454.3 and P3465.1 in a doubled haploid (DH) population derived from the cross Hanxuan 10 × Lumai 14. The DH population comprising 150 lines and a historical population consisting of 154 accessions were used in a 6-SFT-A2 marker–trait association analysis. The three haplotypes were significantly associated with thousand-grain weight (TGW) under rainfed conditions. HapIII had a significant positive effect on TGW. There were significant differences between the Hanxuan 10 and Lumai 14 genotypes in both rainfed and irrigated environments. The average TGW of Lumai 14 (HapIII) was higher than that of Hanxuan 10 (HapI). The frequencies of 6-SFT-A2 HapIII in cultivars released at different periods showed that it had been strongly positively selected in breeding programs. The preferred HapIII for TGW occurred at higher frequencies in Gansu, Beijing, Shanxi, and Hebei than other regions in northern China. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-015-0266-9) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4311048
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-43110482015-02-02 Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat Yue, Aiqin Li, Ang Mao, Xinguo Chang, Xiaoping Li, Runzhi Jing, Ruilian Mol Breed Article As a class of water-soluble, fructose-based oligo- and polysaccharides, fructans are major nonstructural carbohydrates and an important carbon source for grain filling in wheat (Triticum aestivum L.). Four enzymes are involved in fructan synthesis in higher plants, and 6-SFT is a key enzyme in fructan biosynthesis. In this study, thirteen single nucleotide polymorphisms were detected in 6-SFT-A2 in 24 wheat accessions, forming three haplotypes. Two cleaved amplified polymorphic sequence markers developed based on polymorphisms at sites 1870(A-G) and 1951(A-G) distinguished the three haplotypes. 6-SFT-A2 was located on chromosome 4A, between markers P2454.3 and P3465.1 in a doubled haploid (DH) population derived from the cross Hanxuan 10 × Lumai 14. The DH population comprising 150 lines and a historical population consisting of 154 accessions were used in a 6-SFT-A2 marker–trait association analysis. The three haplotypes were significantly associated with thousand-grain weight (TGW) under rainfed conditions. HapIII had a significant positive effect on TGW. There were significant differences between the Hanxuan 10 and Lumai 14 genotypes in both rainfed and irrigated environments. The average TGW of Lumai 14 (HapIII) was higher than that of Hanxuan 10 (HapI). The frequencies of 6-SFT-A2 HapIII in cultivars released at different periods showed that it had been strongly positively selected in breeding programs. The preferred HapIII for TGW occurred at higher frequencies in Gansu, Beijing, Shanxi, and Hebei than other regions in northern China. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-015-0266-9) contains supplementary material, which is available to authorized users. Springer Netherlands 2015-01-30 2015 /pmc/articles/PMC4311048/ /pubmed/25653572 http://dx.doi.org/10.1007/s11032-015-0266-9 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Yue, Aiqin
Li, Ang
Mao, Xinguo
Chang, Xiaoping
Li, Runzhi
Jing, Ruilian
Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat
title Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat
title_full Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat
title_fullStr Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat
title_full_unstemmed Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat
title_short Identification and development of a functional marker from 6-SFT-A2 associated with grain weight in wheat
title_sort identification and development of a functional marker from 6-sft-a2 associated with grain weight in wheat
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311048/
https://www.ncbi.nlm.nih.gov/pubmed/25653572
http://dx.doi.org/10.1007/s11032-015-0266-9
work_keys_str_mv AT yueaiqin identificationanddevelopmentofafunctionalmarkerfrom6sfta2associatedwithgrainweightinwheat
AT liang identificationanddevelopmentofafunctionalmarkerfrom6sfta2associatedwithgrainweightinwheat
AT maoxinguo identificationanddevelopmentofafunctionalmarkerfrom6sfta2associatedwithgrainweightinwheat
AT changxiaoping identificationanddevelopmentofafunctionalmarkerfrom6sfta2associatedwithgrainweightinwheat
AT lirunzhi identificationanddevelopmentofafunctionalmarkerfrom6sfta2associatedwithgrainweightinwheat
AT jingruilian identificationanddevelopmentofafunctionalmarkerfrom6sfta2associatedwithgrainweightinwheat