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Comparison of Vibratome and Compresstome sectioning of fresh primate lymphoid and genital tissues for in situ MHC-tetramer and immunofluorescence staining

BACKGROUND: For decades, the Vibratome served as a standard laboratory resource for sectioning fresh and fixed tissues. In skilled hands, high quality and consistent fresh unfixed tissue sections can be produced using a Vibratome but the sectioning procedure is extremely time consuming. In this stud...

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Detalles Bibliográficos
Autores principales: Abdelaal, Hadia M, Kim, Hyeon O, Wagstaff, Reece, Sawahata, Ryoko, Southern, Peter J, Skinner, Pamela J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4318225/
https://www.ncbi.nlm.nih.gov/pubmed/25657614
http://dx.doi.org/10.1186/s12575-014-0012-4