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Structural and Biochemical Studies of a Moderately Thermophilic Exonuclease I from Methylocaldum szegediense

A novel exonuclease, designated as MszExo I, was cloned from Methylocaldum szegediense, a moderately thermophilic methanotroph. It specifically digests single-stranded DNA in the 3ʹ to 5ʹ direction. The protein is composed of 479 amino acids, and it shares 47% sequence identity with E. coli Exo I. T...

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Detalles Bibliográficos
Autores principales: Fei, Li, Tian, SiSi, Moysey, Ruth, Misca, Mihaela, Barker, John J., Smith, Myron A., McEwan, Paul A., Pilka, Ewa S., Crawley, Lauren, Evans, Tom, Sun, Dapeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4319927/
https://www.ncbi.nlm.nih.gov/pubmed/25658953
http://dx.doi.org/10.1371/journal.pone.0117470