Identification of a Histidine Metal Ligand in the argE-Encoded N-Acetyl-L-Ornithine Deacetylase from Escherichia coli

The H355A, H355K, H80A, and H80K mutant enzymes of the argE-encoded N-acetyl-L-ornithine deacetylase (ArgE) from Escherichia coli were prepared, however, only the H355A enzyme was found to be soluble. Kinetic analysis of the Co(II)-loaded H355A exhibited activity levels that were 380-fold less than...

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Detalles Bibliográficos
Autores principales: McGregor, Wade C, Gillner, Danuta M, Swierczek, Sabina I, Liu, Dali, Holz, Richard C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2013
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320195/
https://www.ncbi.nlm.nih.gov/pubmed/25674394
http://dx.doi.org/10.1186/2193-1801-2-482

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320195/
https://www.ncbi.nlm.nih.gov/pubmed/25674394
http://dx.doi.org/10.1186/2193-1801-2-482

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