Imaging the Drosophila retina: zwitterionic buffers PIPES and HEPES induce morphological artifacts in tissue fixation

BACKGROUND: Tissue fixation is crucial for preserving the morphology of biological structures and cytological details to prevent postmortem degradation and autolysis. Improper fixation conditions could lead to artifacts and thus incorrect conclusions in immunofluorescence or histology experiments. T...

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Detalles Bibliográficos
Autores principales: Nie, Jing, Mahato, Simpla, Zelhof, Andrew C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320506/
https://www.ncbi.nlm.nih.gov/pubmed/25645690
http://dx.doi.org/10.1186/s12861-015-0056-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320506/
https://www.ncbi.nlm.nih.gov/pubmed/25645690
http://dx.doi.org/10.1186/s12861-015-0056-y

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