GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

CRISPR RNA-guided nucleases (RGNs) are widely used genome-editing reagents, but methods to delineate their genome-wide off-target cleavage activities have been lacking. Here we describe an approach for global detection of DNA double-stranded breaks (DSBs) introduced by RGNs and potentially other nuc...

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Detalles Bibliográficos
Autores principales: Tsai, Shengdar Q., Zheng, Zongli, Nguyen, Nhu T., Liebers, Matthew, Topkar, Ved V., Thapar, Vishal, Wyvekens, Nicolas, Khayter, Cyd, Iafrate, A. John, Le, Long P., Aryee, Martin J., Joung, J. Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320685/
https://www.ncbi.nlm.nih.gov/pubmed/25513782
http://dx.doi.org/10.1038/nbt.3117

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320685/
https://www.ncbi.nlm.nih.gov/pubmed/25513782
http://dx.doi.org/10.1038/nbt.3117

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