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Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus

DNA replication in bacteria is accomplished by a multicomponent replicase, the DNA polymerase III holoenzyme (pol III HE). The three essential components of the pol III HE are the α polymerase, the β sliding clamp processivity factor, and the DnaX clamp-loader complex. We report here the assembly of...

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Detalles Bibliográficos
Autores principales: Ribble, Wendy, Kane, Shawn D., Bullard, James M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320859/
https://www.ncbi.nlm.nih.gov/pubmed/25688300
http://dx.doi.org/10.1155/2015/837842