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Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus
DNA replication in bacteria is accomplished by a multicomponent replicase, the DNA polymerase III holoenzyme (pol III HE). The three essential components of the pol III HE are the α polymerase, the β sliding clamp processivity factor, and the DnaX clamp-loader complex. We report here the assembly of...
Autores principales: | Ribble, Wendy, Kane, Shawn D., Bullard, James M. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4320859/ https://www.ncbi.nlm.nih.gov/pubmed/25688300 http://dx.doi.org/10.1155/2015/837842 |
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