Live cell immunogold labelling of RNA polymerase II

Labeling nuclear proteins with electron dense probes in living cells has been a major challenge due to their inability to penetrate into nuclei. We developed a lipid-based approach for delivering antibodies coupled to 0.8 nm ultrasmall gold particles into the nucleus to label RNA polymerase II. Focu...

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Detalles Bibliográficos
Autores principales: Orlov, Igor, Schertel, Andreas, Zuber, Guy, Klaholz, Bruno, Drillien, Robert, Weiss, Etienne, Schultz, Patrick, Spehner, Danièle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321181/
https://www.ncbi.nlm.nih.gov/pubmed/25662860
http://dx.doi.org/10.1038/srep08324

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4321181/
https://www.ncbi.nlm.nih.gov/pubmed/25662860
http://dx.doi.org/10.1038/srep08324

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