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Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells

The reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is lengthy and inefficient. The development of a reprogramming system that allows rapid and synchronous reprogramming to pluripotency is imperative for understanding the mechanism of iPSC formation and for future therapeuti...

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Detalles Bibliográficos
Autores principales: Di Stefano, Bruno, Collombet, Samuel, Graf, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322579/
https://www.ncbi.nlm.nih.gov/pubmed/25977766
http://dx.doi.org/10.1038/sdata.2014.8
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author Di Stefano, Bruno
Collombet, Samuel
Graf, Thomas
author_facet Di Stefano, Bruno
Collombet, Samuel
Graf, Thomas
author_sort Di Stefano, Bruno
collection PubMed
description The reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is lengthy and inefficient. The development of a reprogramming system that allows rapid and synchronous reprogramming to pluripotency is imperative for understanding the mechanism of iPSC formation and for future therapeutic applications. We have recently reported that a short expression in mouse primary B cells of the transcription factor C/EBPα before the induction of pluripotency factors increases the iPSC reprogramming efficiency >100-fold, involving 95% of the cells within a week. Here we present a dataset containing the time course of gene expression during this process as determined by microarray and RNA-seq techniques.
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spelling pubmed-43225792015-05-14 Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells Di Stefano, Bruno Collombet, Samuel Graf, Thomas Sci Data Data Descriptor The reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) is lengthy and inefficient. The development of a reprogramming system that allows rapid and synchronous reprogramming to pluripotency is imperative for understanding the mechanism of iPSC formation and for future therapeutic applications. We have recently reported that a short expression in mouse primary B cells of the transcription factor C/EBPα before the induction of pluripotency factors increases the iPSC reprogramming efficiency >100-fold, involving 95% of the cells within a week. Here we present a dataset containing the time course of gene expression during this process as determined by microarray and RNA-seq techniques. Nature Publishing Group 2014-05-27 /pmc/articles/PMC4322579/ /pubmed/25977766 http://dx.doi.org/10.1038/sdata.2014.8 Text en Copyright © 2014, Macmillan Publishers Limited http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc/3.0/ Metadata associated with this Data Descriptor is available at http://www.nature.com/sdata/ and is released under the CC0 waiver to maximize reuse.
spellingShingle Data Descriptor
Di Stefano, Bruno
Collombet, Samuel
Graf, Thomas
Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells
title Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells
title_full Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells
title_fullStr Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells
title_full_unstemmed Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells
title_short Time-resolved gene expression profiling during reprogramming of C/EBPα-pulsed B cells into iPS cells
title_sort time-resolved gene expression profiling during reprogramming of c/ebpα-pulsed b cells into ips cells
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4322579/
https://www.ncbi.nlm.nih.gov/pubmed/25977766
http://dx.doi.org/10.1038/sdata.2014.8
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