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Construction of three new Gateway(®) expression plasmids for Trypanosoma cruzi

We present here three expression plasmids for Trypanosoma cruzi adapted to the Gateway(®) recombination cloning system. Two of these plasmids were designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag). The TAPtag and Gateway(®) cassette were introd...

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Detalles Bibliográficos
Autores principales: Alonso, Victoria L, Ritagliati, Carla, Cribb, Pamela, Serra, Esteban C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325611/
https://www.ncbi.nlm.nih.gov/pubmed/25424446
http://dx.doi.org/10.1590/0074-0276140238