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Construction of three new Gateway(®) expression plasmids for Trypanosoma cruzi
We present here three expression plasmids for Trypanosoma cruzi adapted to the Gateway(®) recombination cloning system. Two of these plasmids were designed to express trypanosomal proteins fused to a double tag for tandem affinity purification (TAPtag). The TAPtag and Gateway(®) cassette were introd...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4325611/ https://www.ncbi.nlm.nih.gov/pubmed/25424446 http://dx.doi.org/10.1590/0074-0276140238 |