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Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H(2)O(2), TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes

BACKGROUND: Adipose cells responsible for fat storage are the targets of reactive oxygen species (ROS) like H(2)O(2) and pro-inflammatory agents including TNFα and LPS. Such mediators contribute to oxidative stress and alter inflammatory processes in adipose tissue, leading to insulin resistance dur...

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Autores principales: Marimoutou, Méry, Le Sage, Fanny, Smadja, Jacqueline, Lefebvre d’Hellencourt, Christian, Gonthier, Marie-Paule, Robert-Da Silva, Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329220/
https://www.ncbi.nlm.nih.gov/pubmed/25685071
http://dx.doi.org/10.1186/s12950-015-0055-6
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author Marimoutou, Méry
Le Sage, Fanny
Smadja, Jacqueline
Lefebvre d’Hellencourt, Christian
Gonthier, Marie-Paule
Robert-Da Silva, Christine
author_facet Marimoutou, Méry
Le Sage, Fanny
Smadja, Jacqueline
Lefebvre d’Hellencourt, Christian
Gonthier, Marie-Paule
Robert-Da Silva, Christine
author_sort Marimoutou, Méry
collection PubMed
description BACKGROUND: Adipose cells responsible for fat storage are the targets of reactive oxygen species (ROS) like H(2)O(2) and pro-inflammatory agents including TNFα and LPS. Such mediators contribute to oxidative stress and alter inflammatory processes in adipose tissue, leading to insulin resistance during obesity. Thus, the identification of natural compounds such as plant polyphenols able to increase the antioxidant and anti-inflammatory capacity of the body is of high interest. We aimed to evaluate the biological properties of polyphenol-rich extracts from the medicinal plants A. borbonica, D. apetalum and G. mauritiana on preadipocytes exposed to H(2)O(2), TNFα or LPS mediators. METHODS: Medicinal plant extracts were analysed for their polyphenol contents by Folin-Ciocalteu and UPLC-ESI-MS methods as well as for their free radical-scavenging activities by DPPH and ORAC assays. To assess the ability of polyphenol-rich extracts to protect 3T3-L1 preadipocytes against H(2)O(2), TNFα or LPS mediators, several parameters including cell viability (MTT and LDH assays), ROS production (DCFH-DA test), IL-6 and MCP-1 secretion (ELISA) were evaluated. Moreover, the expression of superoxide dismutase, catalase and NF-κB genes was explored (RT-QPCR). RESULTS: All medicinal plants exhibited high levels of polyphenols with free radical-scavenging capacities. Flavonoids such as quercetin, kaempferol, epicatechin and procyanidins, and phenolic acids derived from caffeic acid including chlorogenic acid, were detected. Polyphenol-rich plant extracts did not exert a cytotoxic effect on preadipocytes but protected them against H(2)O(2) anti-proliferative action. Importantly, they down-regulated ROS production and the secretion of IL-6 and MCP-1 pro-inflammatory markers induced by H(2)O(2), TNFα and LPS mediators. Such a protective action was associated with an increase in superoxide dismutase antioxidant enzyme gene expression and a decrease in mRNA levels of NF-κB pro-inflammatory transcription factor. CONCLUSION: This study highlights that antioxidant strategies based on polyphenols derived from medicinal plants tested could contribute to regulate adipose tissue redox status and immune process, and thus participate to the improvement of obesity-related oxidative stress and inflammation.
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spelling pubmed-43292202015-02-16 Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H(2)O(2), TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes Marimoutou, Méry Le Sage, Fanny Smadja, Jacqueline Lefebvre d’Hellencourt, Christian Gonthier, Marie-Paule Robert-Da Silva, Christine J Inflamm (Lond) Research BACKGROUND: Adipose cells responsible for fat storage are the targets of reactive oxygen species (ROS) like H(2)O(2) and pro-inflammatory agents including TNFα and LPS. Such mediators contribute to oxidative stress and alter inflammatory processes in adipose tissue, leading to insulin resistance during obesity. Thus, the identification of natural compounds such as plant polyphenols able to increase the antioxidant and anti-inflammatory capacity of the body is of high interest. We aimed to evaluate the biological properties of polyphenol-rich extracts from the medicinal plants A. borbonica, D. apetalum and G. mauritiana on preadipocytes exposed to H(2)O(2), TNFα or LPS mediators. METHODS: Medicinal plant extracts were analysed for their polyphenol contents by Folin-Ciocalteu and UPLC-ESI-MS methods as well as for their free radical-scavenging activities by DPPH and ORAC assays. To assess the ability of polyphenol-rich extracts to protect 3T3-L1 preadipocytes against H(2)O(2), TNFα or LPS mediators, several parameters including cell viability (MTT and LDH assays), ROS production (DCFH-DA test), IL-6 and MCP-1 secretion (ELISA) were evaluated. Moreover, the expression of superoxide dismutase, catalase and NF-κB genes was explored (RT-QPCR). RESULTS: All medicinal plants exhibited high levels of polyphenols with free radical-scavenging capacities. Flavonoids such as quercetin, kaempferol, epicatechin and procyanidins, and phenolic acids derived from caffeic acid including chlorogenic acid, were detected. Polyphenol-rich plant extracts did not exert a cytotoxic effect on preadipocytes but protected them against H(2)O(2) anti-proliferative action. Importantly, they down-regulated ROS production and the secretion of IL-6 and MCP-1 pro-inflammatory markers induced by H(2)O(2), TNFα and LPS mediators. Such a protective action was associated with an increase in superoxide dismutase antioxidant enzyme gene expression and a decrease in mRNA levels of NF-κB pro-inflammatory transcription factor. CONCLUSION: This study highlights that antioxidant strategies based on polyphenols derived from medicinal plants tested could contribute to regulate adipose tissue redox status and immune process, and thus participate to the improvement of obesity-related oxidative stress and inflammation. BioMed Central 2015-02-08 /pmc/articles/PMC4329220/ /pubmed/25685071 http://dx.doi.org/10.1186/s12950-015-0055-6 Text en © Marimoutou et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Marimoutou, Méry
Le Sage, Fanny
Smadja, Jacqueline
Lefebvre d’Hellencourt, Christian
Gonthier, Marie-Paule
Robert-Da Silva, Christine
Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H(2)O(2), TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes
title Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H(2)O(2), TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes
title_full Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H(2)O(2), TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes
title_fullStr Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H(2)O(2), TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes
title_full_unstemmed Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H(2)O(2), TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes
title_short Antioxidant polyphenol-rich extracts from the medicinal plants Antirhea borbonica, Doratoxylon apetalum and Gouania mauritiana protect 3T3-L1 preadipocytes against H(2)O(2), TNFα and LPS inflammatory mediators by regulating the expression of superoxide dismutase and NF-κB genes
title_sort antioxidant polyphenol-rich extracts from the medicinal plants antirhea borbonica, doratoxylon apetalum and gouania mauritiana protect 3t3-l1 preadipocytes against h(2)o(2), tnfα and lps inflammatory mediators by regulating the expression of superoxide dismutase and nf-κb genes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4329220/
https://www.ncbi.nlm.nih.gov/pubmed/25685071
http://dx.doi.org/10.1186/s12950-015-0055-6
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