Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used to disrupt, correct or insert transgenes at precise locations in mammalian genomes. We demonstrate efficient ‘knock-in’ targeted replacement of multi-kilobase genes in human induced pluripotent stem cells (iPS...

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Detalles Bibliográficos
Autores principales: Byrne, Susan M., Ortiz, Luis, Mali, Prashant, Aach, John, Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330342/
https://www.ncbi.nlm.nih.gov/pubmed/25414332
http://dx.doi.org/10.1093/nar/gku1246

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330342/
https://www.ncbi.nlm.nih.gov/pubmed/25414332
http://dx.doi.org/10.1093/nar/gku1246

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