DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27

Recently, it was reported that knockdown of DICER reduced the ATM-dependent DNA damage response and homologous recombination repair (HRR) via decreasing DICER-generated small RNAs at the damage sites. However, we found that knockdown of DICER dramatically increased cell resistance to camptothecin th...

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Detalles Bibliográficos
Autores principales: Liu, Bailong, Liu, Min, Wang, Jian, Zhang, Xiangming, Wang, Xiang, Wang, Ping, Wang, Hongyan, Li, Wei, Wang, Ya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330351/
https://www.ncbi.nlm.nih.gov/pubmed/25578966
http://dx.doi.org/10.1093/nar/gku1368
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author Liu, Bailong
Liu, Min
Wang, Jian
Zhang, Xiangming
Wang, Xiang
Wang, Ping
Wang, Hongyan
Li, Wei
Wang, Ya
author_facet Liu, Bailong
Liu, Min
Wang, Jian
Zhang, Xiangming
Wang, Xiang
Wang, Ping
Wang, Hongyan
Li, Wei
Wang, Ya
author_sort Liu, Bailong
collection PubMed
description Recently, it was reported that knockdown of DICER reduced the ATM-dependent DNA damage response and homologous recombination repair (HRR) via decreasing DICER-generated small RNAs at the damage sites. However, we found that knockdown of DICER dramatically increased cell resistance to camptothecin that induced damage required ATM to facilitate HRR. This phenotype is due to a prolonged G1/S transition via decreasing DICER-dependent biogenesis of miRNA let-7, which increased the p21(Waf1/Cip1)/p27(Kip1) levels and resulted in decreasing the HRR efficiency. These results uncover a novel function of DICER in regulating the cell cycle through miRNA biogenesis, thus affecting cell response to DNA damage.
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spelling pubmed-43303512015-03-18 DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27 Liu, Bailong Liu, Min Wang, Jian Zhang, Xiangming Wang, Xiang Wang, Ping Wang, Hongyan Li, Wei Wang, Ya Nucleic Acids Res Genome Integrity, Repair and Replication Recently, it was reported that knockdown of DICER reduced the ATM-dependent DNA damage response and homologous recombination repair (HRR) via decreasing DICER-generated small RNAs at the damage sites. However, we found that knockdown of DICER dramatically increased cell resistance to camptothecin that induced damage required ATM to facilitate HRR. This phenotype is due to a prolonged G1/S transition via decreasing DICER-dependent biogenesis of miRNA let-7, which increased the p21(Waf1/Cip1)/p27(Kip1) levels and resulted in decreasing the HRR efficiency. These results uncover a novel function of DICER in regulating the cell cycle through miRNA biogenesis, thus affecting cell response to DNA damage. Oxford University Press 2015-02-18 2015-01-10 /pmc/articles/PMC4330351/ /pubmed/25578966 http://dx.doi.org/10.1093/nar/gku1368 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Genome Integrity, Repair and Replication
Liu, Bailong
Liu, Min
Wang, Jian
Zhang, Xiangming
Wang, Xiang
Wang, Ping
Wang, Hongyan
Li, Wei
Wang, Ya
DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27
title DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27
title_full DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27
title_fullStr DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27
title_full_unstemmed DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27
title_short DICER-dependent biogenesis of let-7 miRNAs affects human cell response to DNA damage via targeting p21/p27
title_sort dicer-dependent biogenesis of let-7 mirnas affects human cell response to dna damage via targeting p21/p27
topic Genome Integrity, Repair and Replication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330351/
https://www.ncbi.nlm.nih.gov/pubmed/25578966
http://dx.doi.org/10.1093/nar/gku1368
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