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Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes

Diffraction limit is usually a thorny problem in an optical inspection system. In this investigation, a model-based deconvolution technique was developed to recover diffraction-limited images, where images with sizes smaller than the diffraction limit could be recognized. Experiments were carried ou...

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Detalles Bibliográficos
Autores principales: Lu, Wenlong, Chang, Ming, Chen, Po-Cheng, Luo, Wun-Mao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330817/
https://www.ncbi.nlm.nih.gov/pubmed/25774075
http://dx.doi.org/10.1080/09500340.2014.924596
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author Lu, Wenlong
Chang, Ming
Chen, Po-Cheng
Luo, Wun-Mao
author_facet Lu, Wenlong
Chang, Ming
Chen, Po-Cheng
Luo, Wun-Mao
author_sort Lu, Wenlong
collection PubMed
description Diffraction limit is usually a thorny problem in an optical inspection system. In this investigation, a model-based deconvolution technique was developed to recover diffraction-limited images, where images with sizes smaller than the diffraction limit could be recognized. Experiments were carried out with a traditional microscope at 200× magnification coupled with a halogen light source for a series of line width samples. The point spread function of the imaging optics was first obtained from an estimated model and then combined with a nonlinear deconvolution algorithm to calculate the full width at half maximum and reconstruct the line widths. Experimental results indicate that a measurement error below one pixel size of the measurement system is achievable. Accordingly, the target of nanoscale line width inspection based on a low cost and real-time image processing technique can be fulfilled, which greatly increases the ability of nanoscaling on optical microscopes.
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spelling pubmed-43308172015-03-12 Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes Lu, Wenlong Chang, Ming Chen, Po-Cheng Luo, Wun-Mao J Mod Opt Research Articles Diffraction limit is usually a thorny problem in an optical inspection system. In this investigation, a model-based deconvolution technique was developed to recover diffraction-limited images, where images with sizes smaller than the diffraction limit could be recognized. Experiments were carried out with a traditional microscope at 200× magnification coupled with a halogen light source for a series of line width samples. The point spread function of the imaging optics was first obtained from an estimated model and then combined with a nonlinear deconvolution algorithm to calculate the full width at half maximum and reconstruct the line widths. Experimental results indicate that a measurement error below one pixel size of the measurement system is achievable. Accordingly, the target of nanoscale line width inspection based on a low cost and real-time image processing technique can be fulfilled, which greatly increases the ability of nanoscaling on optical microscopes. Taylor & Francis 2014-12-12 2014-06-19 /pmc/articles/PMC4330817/ /pubmed/25774075 http://dx.doi.org/10.1080/09500340.2014.924596 Text en © 2014 The Author(s). Published by Taylor & Francis This is an Open Access article. Non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly attributed, cited, and is not altered, transformed, or built upon in any way, is permitted. The moral rights of the named author(s) have been asserted.
spellingShingle Research Articles
Lu, Wenlong
Chang, Ming
Chen, Po-Cheng
Luo, Wun-Mao
Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes
title Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes
title_full Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes
title_fullStr Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes
title_full_unstemmed Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes
title_short Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes
title_sort iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330817/
https://www.ncbi.nlm.nih.gov/pubmed/25774075
http://dx.doi.org/10.1080/09500340.2014.924596
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