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Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes
Diffraction limit is usually a thorny problem in an optical inspection system. In this investigation, a model-based deconvolution technique was developed to recover diffraction-limited images, where images with sizes smaller than the diffraction limit could be recognized. Experiments were carried ou...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330817/ https://www.ncbi.nlm.nih.gov/pubmed/25774075 http://dx.doi.org/10.1080/09500340.2014.924596 |
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author | Lu, Wenlong Chang, Ming Chen, Po-Cheng Luo, Wun-Mao |
author_facet | Lu, Wenlong Chang, Ming Chen, Po-Cheng Luo, Wun-Mao |
author_sort | Lu, Wenlong |
collection | PubMed |
description | Diffraction limit is usually a thorny problem in an optical inspection system. In this investigation, a model-based deconvolution technique was developed to recover diffraction-limited images, where images with sizes smaller than the diffraction limit could be recognized. Experiments were carried out with a traditional microscope at 200× magnification coupled with a halogen light source for a series of line width samples. The point spread function of the imaging optics was first obtained from an estimated model and then combined with a nonlinear deconvolution algorithm to calculate the full width at half maximum and reconstruct the line widths. Experimental results indicate that a measurement error below one pixel size of the measurement system is achievable. Accordingly, the target of nanoscale line width inspection based on a low cost and real-time image processing technique can be fulfilled, which greatly increases the ability of nanoscaling on optical microscopes. |
format | Online Article Text |
id | pubmed-4330817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-43308172015-03-12 Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes Lu, Wenlong Chang, Ming Chen, Po-Cheng Luo, Wun-Mao J Mod Opt Research Articles Diffraction limit is usually a thorny problem in an optical inspection system. In this investigation, a model-based deconvolution technique was developed to recover diffraction-limited images, where images with sizes smaller than the diffraction limit could be recognized. Experiments were carried out with a traditional microscope at 200× magnification coupled with a halogen light source for a series of line width samples. The point spread function of the imaging optics was first obtained from an estimated model and then combined with a nonlinear deconvolution algorithm to calculate the full width at half maximum and reconstruct the line widths. Experimental results indicate that a measurement error below one pixel size of the measurement system is achievable. Accordingly, the target of nanoscale line width inspection based on a low cost and real-time image processing technique can be fulfilled, which greatly increases the ability of nanoscaling on optical microscopes. Taylor & Francis 2014-12-12 2014-06-19 /pmc/articles/PMC4330817/ /pubmed/25774075 http://dx.doi.org/10.1080/09500340.2014.924596 Text en © 2014 The Author(s). Published by Taylor & Francis This is an Open Access article. Non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly attributed, cited, and is not altered, transformed, or built upon in any way, is permitted. The moral rights of the named author(s) have been asserted. |
spellingShingle | Research Articles Lu, Wenlong Chang, Ming Chen, Po-Cheng Luo, Wun-Mao Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes |
title | Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes |
title_full | Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes |
title_fullStr | Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes |
title_full_unstemmed | Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes |
title_short | Iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes |
title_sort | iterative deconvolution technique for measurements of diffraction-limited images on optical microscopes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4330817/ https://www.ncbi.nlm.nih.gov/pubmed/25774075 http://dx.doi.org/10.1080/09500340.2014.924596 |
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