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Development and Validation of an Internationally-Standardized, High-Resolution Capillary Gel-Based Electrophoresis PCR-Ribotyping Protocol for Clostridium difficile

PCR-ribotyping has been adopted in many laboratories as the method of choice for C. difficile typing and surveillance. However, issues with the conventional agarose gel-based technique, including inter-laboratory variation and interpretation of banding patterns have impeded progress. The method has...

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Detalles Bibliográficos
Autores principales: Fawley, Warren N., Knetsch, C. W., MacCannell, Duncan R., Harmanus, Celine, Du, Tim, Mulvey, Michael R., Paulick, Ashley, Anderson, Lydia, Kuijper, E. J., Wilcox, Mark H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4332677/
https://www.ncbi.nlm.nih.gov/pubmed/25679978
http://dx.doi.org/10.1371/journal.pone.0118150