Disclosing Bias in Bisulfite Assay: MethPrimers Underestimate High DNA Methylation

Discordant results obtained in bisulfite assays using MethPrimers (PCR primers designed using MethPrimer software or assuming that non-CpGs cytosines are non methylated) versus primers insensitive to cytosine methylation lead us to hypothesize a technical bias. We therefore used the two kinds of pri...

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Detalles Bibliográficos
Autores principales: Fuso, Andrea, Ferraguti, Giampiero, Scarpa, Sigfrido, Ferrer, Isidre, Lucarelli, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333220/
https://www.ncbi.nlm.nih.gov/pubmed/25692551
http://dx.doi.org/10.1371/journal.pone.0118318

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4333220/
https://www.ncbi.nlm.nih.gov/pubmed/25692551
http://dx.doi.org/10.1371/journal.pone.0118318

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