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Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation
Wheat kernels contain fructans, fructose based oligosaccharides with prebiotic properties, in levels between 2 and 35 weight % depending on the developmental stage of the kernel. To improve knowledge on the metabolic pathways leading to fructan storage and degradation, carbohydrate fluxes occurring...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335405/ https://www.ncbi.nlm.nih.gov/pubmed/25750648 http://dx.doi.org/10.3389/fpls.2015.00089 |
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author | Cimini, Sara Locato, Vittoria Vergauwen, Rudy Paradiso, Annalisa Cecchini, Cristina Vandenpoel, Liesbeth Verspreet, Joran Courtin, Christophe M. D'Egidio, Maria Grazia Van den Ende, Wim De Gara, Laura |
author_facet | Cimini, Sara Locato, Vittoria Vergauwen, Rudy Paradiso, Annalisa Cecchini, Cristina Vandenpoel, Liesbeth Verspreet, Joran Courtin, Christophe M. D'Egidio, Maria Grazia Van den Ende, Wim De Gara, Laura |
author_sort | Cimini, Sara |
collection | PubMed |
description | Wheat kernels contain fructans, fructose based oligosaccharides with prebiotic properties, in levels between 2 and 35 weight % depending on the developmental stage of the kernel. To improve knowledge on the metabolic pathways leading to fructan storage and degradation, carbohydrate fluxes occurring during durum wheat kernel development were analyzed. Kernels were collected at various developmental stages and quali-quantitative analysis of carbohydrates (mono- and di-saccharides, fructans, starch) was performed, alongside analysis of the activities and gene expression of the enzymes involved in their biosynthesis and hydrolysis. High resolution HPAEC-PAD of fructan contained in durum wheat kernels revealed that fructan content is higher at the beginning of kernel development, when fructans with higher DP, such as bifurcose and 1,1-nystose, were mainly found. The changes in fructan pool observed during kernel maturation might be part of the signaling pathways influencing carbohydrate metabolism and storage in wheat kernels during development. During the first developmental stages fructan accumulation may contribute to make kernels more effective Suc sinks and to participate in osmotic regulation while the observed decrease in their content may mark the transition to later developmental stages, transition that is also orchestrated by changes in redox balance. |
format | Online Article Text |
id | pubmed-4335405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-43354052015-03-06 Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation Cimini, Sara Locato, Vittoria Vergauwen, Rudy Paradiso, Annalisa Cecchini, Cristina Vandenpoel, Liesbeth Verspreet, Joran Courtin, Christophe M. D'Egidio, Maria Grazia Van den Ende, Wim De Gara, Laura Front Plant Sci Plant Science Wheat kernels contain fructans, fructose based oligosaccharides with prebiotic properties, in levels between 2 and 35 weight % depending on the developmental stage of the kernel. To improve knowledge on the metabolic pathways leading to fructan storage and degradation, carbohydrate fluxes occurring during durum wheat kernel development were analyzed. Kernels were collected at various developmental stages and quali-quantitative analysis of carbohydrates (mono- and di-saccharides, fructans, starch) was performed, alongside analysis of the activities and gene expression of the enzymes involved in their biosynthesis and hydrolysis. High resolution HPAEC-PAD of fructan contained in durum wheat kernels revealed that fructan content is higher at the beginning of kernel development, when fructans with higher DP, such as bifurcose and 1,1-nystose, were mainly found. The changes in fructan pool observed during kernel maturation might be part of the signaling pathways influencing carbohydrate metabolism and storage in wheat kernels during development. During the first developmental stages fructan accumulation may contribute to make kernels more effective Suc sinks and to participate in osmotic regulation while the observed decrease in their content may mark the transition to later developmental stages, transition that is also orchestrated by changes in redox balance. Frontiers Media S.A. 2015-02-20 /pmc/articles/PMC4335405/ /pubmed/25750648 http://dx.doi.org/10.3389/fpls.2015.00089 Text en Copyright © 2015 Cimini, Locato, Vergauwen, Paradiso, Cecchini, Vandenpoel, Verspreet, Courtin, D'Egidio, Van den Ende and De Gara. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Cimini, Sara Locato, Vittoria Vergauwen, Rudy Paradiso, Annalisa Cecchini, Cristina Vandenpoel, Liesbeth Verspreet, Joran Courtin, Christophe M. D'Egidio, Maria Grazia Van den Ende, Wim De Gara, Laura Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation |
title | Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation |
title_full | Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation |
title_fullStr | Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation |
title_full_unstemmed | Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation |
title_short | Fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation |
title_sort | fructan biosynthesis and degradation as part of plant metabolism controlling sugar fluxes during durum wheat kernel maturation |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4335405/ https://www.ncbi.nlm.nih.gov/pubmed/25750648 http://dx.doi.org/10.3389/fpls.2015.00089 |
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