Proteins interacting with cloning scars: a source of false positive protein-protein interactions

A common approach for exploring the interactome, the network of protein-protein interactions in cells, uses a commercially available ORF library to express affinity tagged bait proteins; these can be expressed in cells and endogenous cellular proteins that copurify with the bait can be identified as...

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Detalles Bibliográficos
Autores principales: Banks, Charles A. S., Boanca, Gina, Lee, Zachary T., Florens, Laurence, Washburn, Michael P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336944/
https://www.ncbi.nlm.nih.gov/pubmed/25704442
http://dx.doi.org/10.1038/srep08530

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336944/
https://www.ncbi.nlm.nih.gov/pubmed/25704442
http://dx.doi.org/10.1038/srep08530

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