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Proteins interacting with cloning scars: a source of false positive protein-protein interactions

A common approach for exploring the interactome, the network of protein-protein interactions in cells, uses a commercially available ORF library to express affinity tagged bait proteins; these can be expressed in cells and endogenous cellular proteins that copurify with the bait can be identified as...

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Detalles Bibliográficos
Autores principales:	Banks, Charles A. S., Boanca, Gina, Lee, Zachary T., Florens, Laurence, Washburn, Michael P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4336944/ https://www.ncbi.nlm.nih.gov/pubmed/25704442 http://dx.doi.org/10.1038/srep08530

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