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Distinct mechanisms regulating mechanical force-induced Ca(2+) signals at the plasma membrane and the ER in human MSCs

It is unclear that how subcellular organelles respond to external mechanical stimuli. Here, we investigated the molecular mechanisms by which mechanical force regulates Ca(2+) signaling at endoplasmic reticulum (ER) in human mesenchymal stem cells. Without extracellular Ca(2+), ER Ca(2+) release is...

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Autores principales: Kim, Tae-Jin, Joo, Chirlmin, Seong, Jihye, Vafabakhsh, Reza, Botvinick, Elliot L, Berns, Michael W, Palmer, Amy E, Wang, Ning, Ha, Taekjip, Jakobsson, Eric, Sun, Jie, Wang, Yingxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337650/
https://www.ncbi.nlm.nih.gov/pubmed/25667984
http://dx.doi.org/10.7554/eLife.04876
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author Kim, Tae-Jin
Joo, Chirlmin
Seong, Jihye
Vafabakhsh, Reza
Botvinick, Elliot L
Berns, Michael W
Palmer, Amy E
Wang, Ning
Ha, Taekjip
Jakobsson, Eric
Sun, Jie
Wang, Yingxiao
author_facet Kim, Tae-Jin
Joo, Chirlmin
Seong, Jihye
Vafabakhsh, Reza
Botvinick, Elliot L
Berns, Michael W
Palmer, Amy E
Wang, Ning
Ha, Taekjip
Jakobsson, Eric
Sun, Jie
Wang, Yingxiao
author_sort Kim, Tae-Jin
collection PubMed
description It is unclear that how subcellular organelles respond to external mechanical stimuli. Here, we investigated the molecular mechanisms by which mechanical force regulates Ca(2+) signaling at endoplasmic reticulum (ER) in human mesenchymal stem cells. Without extracellular Ca(2+), ER Ca(2+) release is the source of intracellular Ca(2+) oscillations induced by laser-tweezer-traction at the plasma membrane, providing a model to study how mechanical stimuli can be transmitted deep inside the cell body. This ER Ca(2+) release upon mechanical stimulation is mediated not only by the mechanical support of cytoskeleton and actomyosin contractility, but also by mechanosensitive Ca(2+) permeable channels on the plasma membrane, specifically TRPM7. However, Ca(2+) influx at the plasma membrane via mechanosensitive Ca(2+) permeable channels is only mediated by the passive cytoskeletal structure but not active actomyosin contractility. Thus, active actomyosin contractility is essential for the response of ER to the external mechanical stimuli, distinct from the mechanical regulation at the plasma membrane. DOI: http://dx.doi.org/10.7554/eLife.04876.001
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spelling pubmed-43376502015-03-04 Distinct mechanisms regulating mechanical force-induced Ca(2+) signals at the plasma membrane and the ER in human MSCs Kim, Tae-Jin Joo, Chirlmin Seong, Jihye Vafabakhsh, Reza Botvinick, Elliot L Berns, Michael W Palmer, Amy E Wang, Ning Ha, Taekjip Jakobsson, Eric Sun, Jie Wang, Yingxiao eLife Biophysics and Structural Biology It is unclear that how subcellular organelles respond to external mechanical stimuli. Here, we investigated the molecular mechanisms by which mechanical force regulates Ca(2+) signaling at endoplasmic reticulum (ER) in human mesenchymal stem cells. Without extracellular Ca(2+), ER Ca(2+) release is the source of intracellular Ca(2+) oscillations induced by laser-tweezer-traction at the plasma membrane, providing a model to study how mechanical stimuli can be transmitted deep inside the cell body. This ER Ca(2+) release upon mechanical stimulation is mediated not only by the mechanical support of cytoskeleton and actomyosin contractility, but also by mechanosensitive Ca(2+) permeable channels on the plasma membrane, specifically TRPM7. However, Ca(2+) influx at the plasma membrane via mechanosensitive Ca(2+) permeable channels is only mediated by the passive cytoskeletal structure but not active actomyosin contractility. Thus, active actomyosin contractility is essential for the response of ER to the external mechanical stimuli, distinct from the mechanical regulation at the plasma membrane. DOI: http://dx.doi.org/10.7554/eLife.04876.001 eLife Sciences Publications, Ltd 2015-02-10 /pmc/articles/PMC4337650/ /pubmed/25667984 http://dx.doi.org/10.7554/eLife.04876 Text en © 2015, Kim et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biophysics and Structural Biology
Kim, Tae-Jin
Joo, Chirlmin
Seong, Jihye
Vafabakhsh, Reza
Botvinick, Elliot L
Berns, Michael W
Palmer, Amy E
Wang, Ning
Ha, Taekjip
Jakobsson, Eric
Sun, Jie
Wang, Yingxiao
Distinct mechanisms regulating mechanical force-induced Ca(2+) signals at the plasma membrane and the ER in human MSCs
title Distinct mechanisms regulating mechanical force-induced Ca(2+) signals at the plasma membrane and the ER in human MSCs
title_full Distinct mechanisms regulating mechanical force-induced Ca(2+) signals at the plasma membrane and the ER in human MSCs
title_fullStr Distinct mechanisms regulating mechanical force-induced Ca(2+) signals at the plasma membrane and the ER in human MSCs
title_full_unstemmed Distinct mechanisms regulating mechanical force-induced Ca(2+) signals at the plasma membrane and the ER in human MSCs
title_short Distinct mechanisms regulating mechanical force-induced Ca(2+) signals at the plasma membrane and the ER in human MSCs
title_sort distinct mechanisms regulating mechanical force-induced ca(2+) signals at the plasma membrane and the er in human mscs
topic Biophysics and Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4337650/
https://www.ncbi.nlm.nih.gov/pubmed/25667984
http://dx.doi.org/10.7554/eLife.04876
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