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Enrichment of CD56(dim)KIR+CD57+ highly cytotoxic NK cells in tumor infiltrated lymph nodes of melanoma patients

An important checkpoint in the progression of melanoma is the metastasis to lymph nodes. Here, to investigate the role of lymph node NK cells in disease progression, we analyze frequency, phenotype and functions of NK cells from tumor-infiltrated (TILN) and tumor-free ipsilateral lymph nodes (TFLN)...

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Autores principales: Ali, Talib Hassan, Pisanti, Simona, Ciaglia, Elena, Mortarini, Roberta, Anichini, Andrea, Garofalo, Cinzia, Tallerico, Rossana, Santinami, Mario, Gulletta, Elio, Ietto, Caterina, Galgani, Mario, Matarese, Giuseppe, Bifulco, Maurizio, Ferrone, Soldano, Colucci, Francesco, Moretta, Alessandro, Kärre, Klas, Carbone, Ennio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338526/
https://www.ncbi.nlm.nih.gov/pubmed/25472612
http://dx.doi.org/10.1038/ncomms6639
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author Ali, Talib Hassan
Pisanti, Simona
Ciaglia, Elena
Mortarini, Roberta
Anichini, Andrea
Garofalo, Cinzia
Tallerico, Rossana
Santinami, Mario
Gulletta, Elio
Ietto, Caterina
Galgani, Mario
Matarese, Giuseppe
Bifulco, Maurizio
Ferrone, Soldano
Colucci, Francesco
Moretta, Alessandro
Kärre, Klas
Carbone, Ennio
author_facet Ali, Talib Hassan
Pisanti, Simona
Ciaglia, Elena
Mortarini, Roberta
Anichini, Andrea
Garofalo, Cinzia
Tallerico, Rossana
Santinami, Mario
Gulletta, Elio
Ietto, Caterina
Galgani, Mario
Matarese, Giuseppe
Bifulco, Maurizio
Ferrone, Soldano
Colucci, Francesco
Moretta, Alessandro
Kärre, Klas
Carbone, Ennio
author_sort Ali, Talib Hassan
collection PubMed
description An important checkpoint in the progression of melanoma is the metastasis to lymph nodes. Here, to investigate the role of lymph node NK cells in disease progression, we analyze frequency, phenotype and functions of NK cells from tumor-infiltrated (TILN) and tumor-free ipsilateral lymph nodes (TFLN) of the same patients. We show an expansion of CD56(dim)CD57(dim)CD69+CCR7+KIR+ NK cells in TILN. TILN NK cells display robust cytotoxic activity against autologous melanoma cells. In the blood of metastatic melanoma patients the frequency of NK cells expressing the receptors for CXCL8 receptor is increased compared to healthy subjects, and blood NK cells also express the receptors for CCL2 and IL6. These factors are produced in high amount in TILN and in vitro switch the phenotype of blood NK cells from healthy donors to the phenotype associated with TILN. Our data suggest that the microenvironment of TILN generates and/or recruits a particularly effective NK cell subset.
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spelling pubmed-43385262015-06-04 Enrichment of CD56(dim)KIR+CD57+ highly cytotoxic NK cells in tumor infiltrated lymph nodes of melanoma patients Ali, Talib Hassan Pisanti, Simona Ciaglia, Elena Mortarini, Roberta Anichini, Andrea Garofalo, Cinzia Tallerico, Rossana Santinami, Mario Gulletta, Elio Ietto, Caterina Galgani, Mario Matarese, Giuseppe Bifulco, Maurizio Ferrone, Soldano Colucci, Francesco Moretta, Alessandro Kärre, Klas Carbone, Ennio Nat Commun Article An important checkpoint in the progression of melanoma is the metastasis to lymph nodes. Here, to investigate the role of lymph node NK cells in disease progression, we analyze frequency, phenotype and functions of NK cells from tumor-infiltrated (TILN) and tumor-free ipsilateral lymph nodes (TFLN) of the same patients. We show an expansion of CD56(dim)CD57(dim)CD69+CCR7+KIR+ NK cells in TILN. TILN NK cells display robust cytotoxic activity against autologous melanoma cells. In the blood of metastatic melanoma patients the frequency of NK cells expressing the receptors for CXCL8 receptor is increased compared to healthy subjects, and blood NK cells also express the receptors for CCL2 and IL6. These factors are produced in high amount in TILN and in vitro switch the phenotype of blood NK cells from healthy donors to the phenotype associated with TILN. Our data suggest that the microenvironment of TILN generates and/or recruits a particularly effective NK cell subset. 2014-12-04 /pmc/articles/PMC4338526/ /pubmed/25472612 http://dx.doi.org/10.1038/ncomms6639 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Ali, Talib Hassan
Pisanti, Simona
Ciaglia, Elena
Mortarini, Roberta
Anichini, Andrea
Garofalo, Cinzia
Tallerico, Rossana
Santinami, Mario
Gulletta, Elio
Ietto, Caterina
Galgani, Mario
Matarese, Giuseppe
Bifulco, Maurizio
Ferrone, Soldano
Colucci, Francesco
Moretta, Alessandro
Kärre, Klas
Carbone, Ennio
Enrichment of CD56(dim)KIR+CD57+ highly cytotoxic NK cells in tumor infiltrated lymph nodes of melanoma patients
title Enrichment of CD56(dim)KIR+CD57+ highly cytotoxic NK cells in tumor infiltrated lymph nodes of melanoma patients
title_full Enrichment of CD56(dim)KIR+CD57+ highly cytotoxic NK cells in tumor infiltrated lymph nodes of melanoma patients
title_fullStr Enrichment of CD56(dim)KIR+CD57+ highly cytotoxic NK cells in tumor infiltrated lymph nodes of melanoma patients
title_full_unstemmed Enrichment of CD56(dim)KIR+CD57+ highly cytotoxic NK cells in tumor infiltrated lymph nodes of melanoma patients
title_short Enrichment of CD56(dim)KIR+CD57+ highly cytotoxic NK cells in tumor infiltrated lymph nodes of melanoma patients
title_sort enrichment of cd56(dim)kir+cd57+ highly cytotoxic nk cells in tumor infiltrated lymph nodes of melanoma patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338526/
https://www.ncbi.nlm.nih.gov/pubmed/25472612
http://dx.doi.org/10.1038/ncomms6639
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