Rapid preparation of high-purity nuclear proteins from a small number of cultured cells for use in electrophoretic mobility shift assays

BACKGROUND: Highly purified nuclear protein is required when using an electrophoretic mobility shift assay (EMSA) to study transcription factors, e.g. nuclear factor-κB (NF-κB), a major transcription factor that regulates both innate and adaptive immune responses following infection. Although many p...

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Detalles Bibliográficos
Autores principales: Luo, Yuqian, Hara, Takeshi, Ishido, Yuko, Yoshihara, Aya, Oda, Kenzaburo, Makino, Masahiko, Ishii, Norihisa, Hiroi, Naoki, Suzuki, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339431/
https://www.ncbi.nlm.nih.gov/pubmed/25527077
http://dx.doi.org/10.1186/s12865-014-0062-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339431/
https://www.ncbi.nlm.nih.gov/pubmed/25527077
http://dx.doi.org/10.1186/s12865-014-0062-z

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