Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway

Chinese hamster ovary (CHO) cells are the most widely used mammalian hosts for production of therapeutic proteins. However, development of recombinant CHO cell lines has been hampered by unstable and variable transgene expression caused by random integration. Here we demonstrate efficient targeted g...

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Detalles Bibliográficos
Autores principales: Lee, Jae Seong, Kallehauge, Thomas Beuchert, Pedersen, Lasse Ebdrup, Kildegaard, Helene Faustrup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339809/
https://www.ncbi.nlm.nih.gov/pubmed/25712033
http://dx.doi.org/10.1038/srep08572

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339809/
https://www.ncbi.nlm.nih.gov/pubmed/25712033
http://dx.doi.org/10.1038/srep08572

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