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Exogenous reference gene normalization for real-time reverse transcription-polymerase chain reaction analysis under dynamic endogenous transcription

Quantitative real-time reverse transcription-polymerase chain reaction (qPCR) is widely used to investigate transcriptional changes following experimental manipulations to the nervous system. Despite the widespread utilization of qPCR, the interpretation of results is marred by the lack of a suitabl...

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Detalles Bibliográficos
Autores principales: Johnston, Stephen, Gallaher, Zachary, Czaja, Krzysztof
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340019/
https://www.ncbi.nlm.nih.gov/pubmed/25722696
http://dx.doi.org/10.3969/j.issn.1673-5374.2012.14.004