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Exogenous reference gene normalization for real-time reverse transcription-polymerase chain reaction analysis under dynamic endogenous transcription
Quantitative real-time reverse transcription-polymerase chain reaction (qPCR) is widely used to investigate transcriptional changes following experimental manipulations to the nervous system. Despite the widespread utilization of qPCR, the interpretation of results is marred by the lack of a suitabl...
Autores principales: | Johnston, Stephen, Gallaher, Zachary, Czaja, Krzysztof |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340019/ https://www.ncbi.nlm.nih.gov/pubmed/25722696 http://dx.doi.org/10.3969/j.issn.1673-5374.2012.14.004 |
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