An Evolved Mxe GyrA Intein for Enhanced Production of Fusion Proteins

[Image: see text] Expressing antibodies as fusions to the non-self-cleaving Mxe GyrA intein enables site-specific, carboxy-terminal chemical modification of the antibodies by expressed protein ligation (EPL). Bacterial antibody-intein fusion protein expression platforms typically yield insoluble inc...

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Detalles Bibliográficos
Autores principales: Marshall, Carrie J., Grosskopf, Vanessa A., Moehling, Taylor J., Tillotson, Benjamin J., Wiepz, Gregory J., Abbott, Nicholas L., Raines, Ronald T., Shusta, Eric V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340354/
https://www.ncbi.nlm.nih.gov/pubmed/25384269
http://dx.doi.org/10.1021/cb500689g

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340354/
https://www.ncbi.nlm.nih.gov/pubmed/25384269
http://dx.doi.org/10.1021/cb500689g

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