How Many proteins are Missed in Quantitative proteomics Based on Ms/Ms sequencing Methods?

Current bottom-up quantitative proteomics methods based on MS/MS sequencing of peptides are shown to be strongly dependent on sample preparation. Using cytosolic proteins from MCF-7 breast cancer cells, it is shown that protein pre-fractionation based on pI and MW is more effective than pre-fraction...

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Detalles Bibliográficos
Autores principales: Mulvey, Claire, Thur, Bettina, Crawford, Mark, Godovac-Zimmermann, Jasminka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340538/
https://www.ncbi.nlm.nih.gov/pubmed/25729266
http://dx.doi.org/10.4137/PRI.S5882

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340538/
https://www.ncbi.nlm.nih.gov/pubmed/25729266
http://dx.doi.org/10.4137/PRI.S5882

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