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How Many proteins are Missed in Quantitative proteomics Based on Ms/Ms sequencing Methods?
Current bottom-up quantitative proteomics methods based on MS/MS sequencing of peptides are shown to be strongly dependent on sample preparation. Using cytosolic proteins from MCF-7 breast cancer cells, it is shown that protein pre-fractionation based on pI and MW is more effective than pre-fraction...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340538/ https://www.ncbi.nlm.nih.gov/pubmed/25729266 http://dx.doi.org/10.4137/PRI.S5882 |