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Measuring protein reduction potentials using (15)N HSQC NMR spectroscopy()

NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple (15)N HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 con...

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Detalles Bibliográficos
Autores principales: Taylor, Samantha L., Crawley-Snowdon, Harriet, Wagstaff, Jane L., Rowe, Michelle L., Shepherd, Mark, Williamson, Richard A., Howard, Mark J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340554/
https://www.ncbi.nlm.nih.gov/pubmed/23360928
http://dx.doi.org/10.1039/c3cc38952a