Measuring protein reduction potentials using (15)N HSQC NMR spectroscopy()

NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple (15)N HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 con...

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Detalles Bibliográficos
Autores principales: Taylor, Samantha L., Crawley-Snowdon, Harriet, Wagstaff, Jane L., Rowe, Michelle L., Shepherd, Mark, Williamson, Richard A., Howard, Mark J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340554/
https://www.ncbi.nlm.nih.gov/pubmed/23360928
http://dx.doi.org/10.1039/c3cc38952a
Descripción
Sumario:NMR spectroscopy was used to measure reduction potentials of four redox proteins by following multiple (15)N HSQC protein resonances across a titration series using mixtures of oxidised and reduced glutathione. Results for PDI a, PDI ab and DsbA agree with the literature and our result for ERp18 confirms this protein as an oxidoreductase of comparable or greater reducing strength than PDI a.

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