Cargando…

Pharmacokinetic Study of 14-(3-Methylbenzyl)matrine and 14-(4-Methylbenzyl)matrine in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry

A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separat...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Minjie, Wang, Lisheng, Huang, Shulin, Xu, Liba, Hu, Chao, Jiang, Weizhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340794/
https://www.ncbi.nlm.nih.gov/pubmed/25714369
http://dx.doi.org/10.1371/journal.pone.0116010
_version_ 1782359057941659648
author Jiang, Minjie
Wang, Lisheng
Huang, Shulin
Xu, Liba
Hu, Chao
Jiang, Weizhe
author_facet Jiang, Minjie
Wang, Lisheng
Huang, Shulin
Xu, Liba
Hu, Chao
Jiang, Weizhe
author_sort Jiang, Minjie
collection PubMed
description A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separated using a C18 column (1.9 μm, 2.1 mm × 100 mm) equipped with a Security Guard C18 column (5 μm, 2.1 mm × 10 mm), followed by detection via triple-quadrupole mass spectrometry using an electrospray ionization (ESI) source. Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard. The method was linear in the concentration range of 5–2000 ng/ml for both compounds. The intra-day and inter-day relative standard deviations (RSDs) were less than 15%, and all relative errors (REs) were within 15%. The proposed method enables the unambiguous identification and quantification of these two compounds in vivo. This study is the first to determine the 3MBM and 4MBM levels in rat plasma after oral administration of these compounds. These results provide a meaningful basis for evaluating the clinical applications of these medicines.
format Online
Article
Text
id pubmed-4340794
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-43407942015-03-04 Pharmacokinetic Study of 14-(3-Methylbenzyl)matrine and 14-(4-Methylbenzyl)matrine in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry Jiang, Minjie Wang, Lisheng Huang, Shulin Xu, Liba Hu, Chao Jiang, Weizhe PLoS One Research Article A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separated using a C18 column (1.9 μm, 2.1 mm × 100 mm) equipped with a Security Guard C18 column (5 μm, 2.1 mm × 10 mm), followed by detection via triple-quadrupole mass spectrometry using an electrospray ionization (ESI) source. Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard. The method was linear in the concentration range of 5–2000 ng/ml for both compounds. The intra-day and inter-day relative standard deviations (RSDs) were less than 15%, and all relative errors (REs) were within 15%. The proposed method enables the unambiguous identification and quantification of these two compounds in vivo. This study is the first to determine the 3MBM and 4MBM levels in rat plasma after oral administration of these compounds. These results provide a meaningful basis for evaluating the clinical applications of these medicines. Public Library of Science 2015-02-25 /pmc/articles/PMC4340794/ /pubmed/25714369 http://dx.doi.org/10.1371/journal.pone.0116010 Text en © 2015 Jiang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jiang, Minjie
Wang, Lisheng
Huang, Shulin
Xu, Liba
Hu, Chao
Jiang, Weizhe
Pharmacokinetic Study of 14-(3-Methylbenzyl)matrine and 14-(4-Methylbenzyl)matrine in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title Pharmacokinetic Study of 14-(3-Methylbenzyl)matrine and 14-(4-Methylbenzyl)matrine in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_full Pharmacokinetic Study of 14-(3-Methylbenzyl)matrine and 14-(4-Methylbenzyl)matrine in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_fullStr Pharmacokinetic Study of 14-(3-Methylbenzyl)matrine and 14-(4-Methylbenzyl)matrine in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_full_unstemmed Pharmacokinetic Study of 14-(3-Methylbenzyl)matrine and 14-(4-Methylbenzyl)matrine in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_short Pharmacokinetic Study of 14-(3-Methylbenzyl)matrine and 14-(4-Methylbenzyl)matrine in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry
title_sort pharmacokinetic study of 14-(3-methylbenzyl)matrine and 14-(4-methylbenzyl)matrine in rat plasma using liquid chromatography-tandem mass spectrometry
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4340794/
https://www.ncbi.nlm.nih.gov/pubmed/25714369
http://dx.doi.org/10.1371/journal.pone.0116010
work_keys_str_mv AT jiangminjie pharmacokineticstudyof143methylbenzylmatrineand144methylbenzylmatrineinratplasmausingliquidchromatographytandemmassspectrometry
AT wanglisheng pharmacokineticstudyof143methylbenzylmatrineand144methylbenzylmatrineinratplasmausingliquidchromatographytandemmassspectrometry
AT huangshulin pharmacokineticstudyof143methylbenzylmatrineand144methylbenzylmatrineinratplasmausingliquidchromatographytandemmassspectrometry
AT xuliba pharmacokineticstudyof143methylbenzylmatrineand144methylbenzylmatrineinratplasmausingliquidchromatographytandemmassspectrometry
AT huchao pharmacokineticstudyof143methylbenzylmatrineand144methylbenzylmatrineinratplasmausingliquidchromatographytandemmassspectrometry
AT jiangweizhe pharmacokineticstudyof143methylbenzylmatrineand144methylbenzylmatrineinratplasmausingliquidchromatographytandemmassspectrometry