N6-Adenosine Methylation in MiRNAs

Methylation of N6-adenosine (m6A) has been observed in many different classes of RNA, but its prevalence in microRNAs (miRNAs) has not yet been studied. Here we show that a knockdown of the m6A demethylase FTO affects the steady-state levels of several miRNAs. Moreover, RNA immunoprecipitation with...

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Autores principales: Berulava, Tea, Rahmann, Sven, Rademacher, Katrin, Klein-Hitpass, Ludgar, Horsthemke, Bernhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344304/
https://www.ncbi.nlm.nih.gov/pubmed/25723394
http://dx.doi.org/10.1371/journal.pone.0118438
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author Berulava, Tea
Rahmann, Sven
Rademacher, Katrin
Klein-Hitpass, Ludgar
Horsthemke, Bernhard
author_facet Berulava, Tea
Rahmann, Sven
Rademacher, Katrin
Klein-Hitpass, Ludgar
Horsthemke, Bernhard
author_sort Berulava, Tea
collection PubMed
description Methylation of N6-adenosine (m6A) has been observed in many different classes of RNA, but its prevalence in microRNAs (miRNAs) has not yet been studied. Here we show that a knockdown of the m6A demethylase FTO affects the steady-state levels of several miRNAs. Moreover, RNA immunoprecipitation with an anti-m6A-antibody followed by RNA-seq revealed that a significant fraction of miRNAs contains m6A. By motif searches we have discovered consensus sequences discriminating between methylated and unmethylated miRNAs. The epigenetic modification of an epigenetic modifier as described here adds a new layer to the complexity of the posttranscriptional regulation of gene expression.
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spelling pubmed-43443042015-03-04 N6-Adenosine Methylation in MiRNAs Berulava, Tea Rahmann, Sven Rademacher, Katrin Klein-Hitpass, Ludgar Horsthemke, Bernhard PLoS One Research Article Methylation of N6-adenosine (m6A) has been observed in many different classes of RNA, but its prevalence in microRNAs (miRNAs) has not yet been studied. Here we show that a knockdown of the m6A demethylase FTO affects the steady-state levels of several miRNAs. Moreover, RNA immunoprecipitation with an anti-m6A-antibody followed by RNA-seq revealed that a significant fraction of miRNAs contains m6A. By motif searches we have discovered consensus sequences discriminating between methylated and unmethylated miRNAs. The epigenetic modification of an epigenetic modifier as described here adds a new layer to the complexity of the posttranscriptional regulation of gene expression. Public Library of Science 2015-02-27 /pmc/articles/PMC4344304/ /pubmed/25723394 http://dx.doi.org/10.1371/journal.pone.0118438 Text en © 2015 Berulava et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Berulava, Tea
Rahmann, Sven
Rademacher, Katrin
Klein-Hitpass, Ludgar
Horsthemke, Bernhard
N6-Adenosine Methylation in MiRNAs
title N6-Adenosine Methylation in MiRNAs
title_full N6-Adenosine Methylation in MiRNAs
title_fullStr N6-Adenosine Methylation in MiRNAs
title_full_unstemmed N6-Adenosine Methylation in MiRNAs
title_short N6-Adenosine Methylation in MiRNAs
title_sort n6-adenosine methylation in mirnas
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4344304/
https://www.ncbi.nlm.nih.gov/pubmed/25723394
http://dx.doi.org/10.1371/journal.pone.0118438
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AT horsthemkebernhard n6adenosinemethylationinmirnas

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