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Spotting effect in microarray experiments

BACKGROUND: Microarray data must be normalized because they suffer from multiple biases. We have identified a source of spatial experimental variability that significantly affects data obtained with Cy3/Cy5 spotted glass arrays. It yields a periodic pattern altering both signal (Cy3/Cy5 ratio) and i...

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Detalles Bibliográficos
Autores principales: Mary-Huard, Tristan, Daudin, Jean-Jacques, Robin, Stéphane, Bitton, Frédérique, Cabannes, Eric, Hilson, Pierre
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC434492/
https://www.ncbi.nlm.nih.gov/pubmed/15151695
http://dx.doi.org/10.1186/1471-2105-5-63